CCRF
// Facilities
CCRF
In an effort to boost innovation and research, a centralized core research facility (CCRF) has been established at the AIIMS, New Delhi. This facility is housed at 9th floor in the Convergence block at AIIMS and encompasses high end instrumentation for Genomics, Proteomics, Bioinformatics, Bioanalytics , BSL-3, Microscopy, Flow cytometry, and other areas. The facility aims to impart a quantum thrust to basic, clinical and translational research in the High priority areas of genomics, proteomics, immunology, infection biology and bioinformatics using state-of-the art platform technologies at AIIMS. The creation of this special Centre would be helpful to place AIIMS at par with the world’s best institutions in these highly competitive and promising fields within a decade.
BSL – 3 Facility
- Class II B2 Type Biosafety Cabinets (BSC)
- CO2 incubators, BOD incubators, Shaker incubators
- Refrigerated Centrifuge, Microfuge, Picofuge
- Anaerobic Glove box
- Micropipettes, Multi-channel pipettes, Pipette aids
- MGIT
- Refrigerators (4 °C), Deep Freezers (-20 °C & -80 °C) and Nitrogen Cylinders for short term storage of biological samples and reagents
- Digital Colony Counter
- LED Microscope
- RT PCR Machine
- Double Door Autoclave and vertical autoclaves for decontamination of the bio-hazardous waste
- Ultracentrifuge
- Nucleic acid Extraction system
- Bead beater and Tissue homogeniser
- Multi-mode Reader
- ELISA Plate Reader
Genomic Facility
- Whole-genome sequencing (WGS)
- Whole-exome sequencing (WES)
- RNA sequencing (RNA-seq)
- Small RNA sequencing (sRNA-seq)
- Copy number variation (CNV)
- Targeted sequencing/panels
- de novo genome assembly and annotation
- Pan-genome (core genome and accessory genome)
- Illumina BeadChip Array (HumanMethylation450/EPIC)
- Microarray (Affymetrix, Agilent, and Illumina)
- 16S/Metagenomics
- Antimicrobial resistance (AMR)
- Gene ontology and pathway enrichment analysis
- Functional gene annotation
- Sequence/genome alignment
- Comparative genomics
- Motif prediction
- Phylogenetic analysis
- Protein-protein interaction network
- Molecular docking and simulation
- Bioinformatics databases and data mining
- Machine learning/AI on Genomics data
- Sequencing on Novaseq 6000*(Illumina)
- Sequencing on Miseq *(Illumina)
- Sequencing on 3730 Genetic analyzer** (Sanger Sequencer, Applied Biosystems)
- Quality check of RNA/ NGS libraries on Fragment Analyzer 5200 (Agilent Technologies)
- Quantification of DNA/ RNA/ NGS Libraries on Qubit (Invitrogen)
- DNA/RNA/Chromatin Shearing on focussed ultrasonicator ME220
- Expression profiling on nCounter Sprint Profiler (Nanostring)
Proteomic Facility
- LCMS based Proteomics (Both qualitative and quantitative)
- Label based LCMS proteomics (4*4 plex, kits have to procured by user)
- Iso-electric focusing
- 2D electrophoresis
- Gel Scanning flow
- High Pressure Liquid Chromatography
- Flash chromatography analysis
General Facility
- Real Time PCR machine
- PCR
- Ultra-Centrifuge
- Imaging system
- Electroporator
- Flow-Cytometer
- Cell Sorter
- Refrigerated Microfuge
- Homogenizer
- Agarose gel electrophoresis
- SDS-PAGE
- Western Blot apparatus
- ELISA Reader and Washer
- Gel Documentation System
- MilliQ & RO water system
- Icematic
- pH Meter
- Magnetic stirrer
- Shacking Incubator
- Water bath cum shaker
// Checkout More About Incubation Charges and Application Process